Coding

Part:BBa_K5301006:Design

Designed by: Xiaowen Sun   Group: iGEM24_BNU-China   (2024-09-22)


SnCSdT is one of the components of multi-polymerized MSP.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1021
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1021
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 203
    Illegal BglII site found at 575
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1021
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1021
    Illegal AgeI site found at 100
    Illegal AgeI site found at 860
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is no cross-reaction between SpyTag/Catcher and SnoopTag/Catcher.


Source

SnoopCatcher is derived from RrgA, an adhesin from Streptococcus pneumoniae. SdyTag is derived from Streptococcus dysgalactiae protein, CnaB. NW15 is the truncated form of NW30, which is originated from a set of α-helixes derived from apolipoprotein A-1 (ApoA1).

References